18 Feb 2008 04:33:57 | Wendy Butler
The compound microscope is one form of microscope that uses a
single glass lens of short focal length for the object while
another single glass lens for the ocular or eyepiece. The modern
compound microscope is generally far more complex in nature.
These deal with multiple lenses. These lenses are on both the
ends i.e. objective and eyepiece assemblies.
Chromatic aberration and spherical aberration appear in a much
more reduced form when these multi-component lenses are used. To
provide stable, controllable illumination the mirror is replaced
by a lamp unit in a modern compound microscope.
It is basically an uncertain criterion while indicating the
inventor of the compound microscope. It is often and generally
believed that in 1590 two persons, father and son,
spectacle-makers from the Netherlands, invented the first
compound microscope. Their names were Hans Janssen and Zacharias
Janssen. But there is a real heavyweight contender for the
title. Galileo Galilei. In 1609 Galileo Galilei developed an
"occhiolino" or compound microscope. It comprised of a convex
and a concave lens. In the late 1600's Christiaan Huygens,
another inventor from the Netherlands also developed a two-lens
or a compound microscope.
But whoever invented it, the compound microscope follows almost
the same principal till date. The compound microscope could be
divided into eight major parts depending on their functionality.
They are ocular lens or eye-piece, objective turret or
nosepiece, objective lenses, coarse adjustment knob, fine
adjustment knob, object holder or stage, mirror and diaphragm
and condenser. Each of the parts synchronizes with the other and
this reveals an image that is magnified up to 1000Ã. A compound
microscope is used on specimens that have a very limited depth
of field i.e. very minute or thin specimens.
A typical compound microscope is composed of three main
objective lenses. The first one is the scanning lens. This one
has a magnifying power of 4×. Then there is a low power lens
with a magnifying power of 10×, and a high power lens with a
magnifying power of 40×. In the more advanced version of the
compound microscope there is an additional lease present. This
fourth objective lens is an oil immersion lens. This oil
immersion lens can champion a magnifying power of about 100×.
According to the Encyclopedia, "Optical microscopes are
restricted in their ability to resolve features by a phenomenon
called diffraction which, based on the numerical aperture (NA or
/A/_/N/) of the optical system and the wavelengths of light used
(λ), sets a definite limit (/d/) to the optical resolution.
Assuming that optical aberrations are negligible, the resolution
(/d/) is given by: d = frac {lambda} {A_N} usually, a λ of 550
nm is assumed, corresponding to green light. With air as medium,
the highest practical /A/_/N/ is 0.95, and with oil, up to 1.5.
Due to diffraction, even the best optical microscope is limited
to a resolution of 0.2 micrometers."
On the whole a compound microscope is the most widely used and
vastly accepted microscope in the world.
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